Optimalisasi Media Gellan Gum untuk Penyimpanan Bakteri: Analisis Viabilitas pada Berbagai Konsentrasi
Date Published : 1 November 2025
Contributors
Nabila Utami
Author
Diah Ayu Prawitasari
Co-author
Annisa Nur Lathifah
Co-author
Keywords
Bioteknologi
Gellan Gum
Isolat Bakteri
Viabilitas
Proceeding
Track
Paper Prosiding Seminar
License
Copyright (c) 2025 Seminar Nasional Teknik Lingkungan (SNTL)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Abstract
Penelitian ini bertujuan untuk mengeksplorasi teknik penyimpanan isolat bakteri dalam media gellan gum dengan variasi konsentrasi 2,5% dan 3%. Viabilitas koloni bakteri dievaluasi dengan metode Total Plate Count (TPC), serta pengamatan morfologi sel diamati melalui teknik pewarnaan gram. Sampel bakteri yang digunakan merupakan koleksi Laboratorium Teknik Lingkungan, Universitas Islam Indonesia, yang memiliki potensi dalam aplikasi bioremediasi dan bioteknologi. Kultur bakteri diinokulasikan ke dalam media semi padat DNB (Dilute Nutrient Broth) ditambah gellan gum dengan konsentrasi 2,5% dan 3%. Hasil penelitian menunjukkan terdapat perbedaan pertumbuhan koloni bakteri yang signifikan antara kedua konsentrasi. Pada konsentrasi 2,5%, jumlah koloni yang terhitung dalam sampel isolat lebih rendah dibandingkan pada konsentrasi 3%. Selain itu, dilakukan pengamatan morfologi sel dengan pengecatan gram. Teknik ini menunjukkan bahwa bakteri yang diuji termasuk gram positif. Pengamatan morfologi makroskopis meliputi bentuk sel, tepi, dan elevasi bakteri yang diteliti, sehingga memberikan informasi tambahan mengenai karakteristik bakteri tersebut. Hasil penelitian menunjukkan bahwa media DNB ditambah gellan gum dengan konsentrasi 3% lebih efektif dalam mendukung pertumbuhan koloni bakteri. Hasil penelitian ini dapat dijadikan alternatif penyimpanan bakteri dalam jangka panjang dengan mempertahankan viabilitas dan morfologi selnya, sehingga berpotensi untuk mendukung aplikasi bioremediasi dan bioteknologi di masa mendatang.
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Utami, N., Prawitasari, D. A., & Lathifah, A. N. (2025). Optimalisasi Media Gellan Gum untuk Penyimpanan Bakteri: Analisis Viabilitas pada Berbagai Konsentrasi. Seminar Nasional Teknik Lingkungan (SNTL), 1, 084-100. https://conference.uii.ac.id/sntl/paper/view/92